Single-molecule fluorescence studies on DNA looping
نویسندگان
چکیده
منابع مشابه
Studying DNA looping by single-molecule FRET.
Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into nucleosomes. Thermodynamics of dsDNA bending has been studied by a method called cyclization which relies on DNA ligase to covalently join short sticky ends of a dsDNA. However, ligation efficiency can be affected by many factors that are not relat...
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ژورنال
عنوان ژورنال: Methods
سال: 2016
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2016.04.005